doTERRA On Guard® essential oil blend contains oils from wild wild orange, clove, cinnamon-bark, eucalyptus en rosemary. It can be used to protect against environmental threats and to support healthy immune function.* A recent study by doTERRA Scientists evaluated the effects of doTERRA On Guard mixture on human cells. Four different white blood cell co-cultures and one skin cell co-culture were exposed to the mixture and subsequent changes in cellular activity were measured by biomarker analysis.
The combination of multiple cell types grown together in the same dish is called a co-culture. Co-cultures are often more useful models of cell activity than single cell lines because they are more similar to the conditions in the human body, which is a complex combination of different cell types. The co-cultures selected for experimentation in this study have been validated by preliminary research as effective models of inflammatory or immunomodulatory processes.1,2
In total, nine different biomarkers were observed that were knocked down in the different white blood cell co-cultures. These were the immunomodulatory markers CD40, sIgG, sIL-17A, sIL-17F, sIL-2 and sIL-6, as well as the inflammatory markers sTNFα, V-CAM1, IP-10, Eotaxin-3 and P-Selectin.3
Biomarkers in the co-culture of the skin cells were also strongly affected. Levels of the inflammation-related markers VCAM-1, IP-10, I-TAC, MIG, PAI-1 and M-CSF decreased after exposure to doTERRA On Guard mixture.3 Cell proliferation also decreased significantly. RNA microarray analysis has shown that gene expression is altered in these cells, with the top four matched signaling pathways related to inflammation and cell cycle control.
These results suggest that the effects of doTERRA On Guard Essential Oil Blend are consistent with the known effects of the individual oils in the blend. The experimental data also suggest that doTERRA On Guard may regulate many important pathways relevant to healthy inflammatory response, wound healing, cellular health and immune response.*
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent disease.