DoTERRA On Guard® essential oil blend contains oils from wild wild orange, clove, cinnamon bark, eucalyptus and rosemary. It can be used to protect against environmental threats and support healthy immune function.* A recent study of doTERRA scientists evaluated the effects of doTERRA On Guard mixture on human cells. Four different co-cultures of white blood cells and a co-culture of skin cells were exposed to the mixture and subsequent changes in cellular activity were measured using biomarker analysis.
The combination of multiple cell types grown together in the same dish is called a co-culture. Co-cultures are often more useful models of cell activity than single cell lines because they more closely resemble conditions in the human body, which is a complex combination of different cell types. The co-cultures selected for experimentation in this study have been validated by prior research as effective models of inflammatory or immunomodulatory processes.1,2
A total of nine different biomarkers were observed to be down-regulated in the different white blood cell co-cultures. These were the immunomodulatory markers CD40, sIgG, sIL-17A, sIL-17F, sIL-2 and sIL-6, as well as the inflammatory markers sTNFα, V-CAM1, IP-10, Eotaxin-3 and P-Selectin.3
Biomarkers in skin cell co-culture were also significantly affected. The levels of inflammation-related markers VCAM-1, IP-10, I-TAC, MIG, PAI-1 and M-CSF decreased after exposure to doTERRA On Guard mixture.3 Cell proliferation also decreased significantly. RNA microarray analysis revealed that gene expression was altered in these cells, with the top four matched signaling pathways being related to inflammation and cell cycle control.
These results suggest that the effects of doTERRA On Guard essential oil blend are consistent with the known effects of the individual oils in the blend. Experimental data also suggest that doTERRA On Guard can regulate many important pathways relevant to a healthy inflammatory response, wound healing, cellular health and immune response.*
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.